ITCH anticorps (AA 17-358)

N° du produit ABIN7600263

Cet anticorps Souris Monoclonal détecte spécifiquement ITCH dans WB, IHC et FACS. Il présente une réactivité envers Humain, Souris et Rat.

Aperçu rapide pour ITCH anticorps (AA 17-358) (ABIN7600263)

Antigène: ITCH (Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH))

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ITCH est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Clone: 5E12

Détail du produit anti-ITCH anticorps

Épitope: AA 17-358

Fonction: Anti-ITCH/AIP4 Antibody Picoband® (monoclonal, 5E12)

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-ITCH/AIP4 Antibody Picoband® (monoclonal, 5E12) (ABIN7600263). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human ITCH/AIP4 recombinant protein (Position: K17-E358).

Isotype: IgG2b

Information d'application

Indications d'application: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x⁶ cells, Human
1. Dorjbal B, Derse D, Lloyd P, Soheilian F, Nagashima K, Heidecker G. ""The role of ITCH protein in human T-cell leukemia virus type 1 release"". J Biol Chem, 2011 Sep 9. 2. Rossi M, Inoue S, Walewska R, Knight RA, Dyer MJ, Cohen GM, Melino G. ""Caspase cleavage of Itch in chronic lymphocytic leukemia cells"". Biochem Biophys Res Commun, 2009 Feb 13.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Détails sur ITCH

Antigène: ITCH (Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH))

Autre désignation: ITCH

Sujet:

Synonyms: Serine/threonine-protein kinase PLK1, Polo-like kinase 1, PLK-1, Serine/threonine-protein kinase 13, STPK13, PLK1, PLK

Tissue Specificity: Placenta and colon.

Background: ITCH is an ubiquitin-conjugating enzyme. This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein plays a role in multiple cellular processes including erythroid and lymphoid cell differentiation and the regulation of immune responses. Mutations in this gene are a cause of syndromic multisystem autoimmune disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Poids moléculaire: 103 kDa

ID gène: 83737

UniProt: Q96J02

Pathways: Activation of Innate immune Response, CXCR4-mediated Signaling Events